English: A z-projection of an osteosarcoma cell, stained with phalloidin to visualise actin filaments. The image was taken on a confocal microscope, and the subsequent deconvolution was done using an experimentally derived point spread function.

Image Parameters
Microscope: Zeiss LSM 780 Confocal Microscope
Pinhole [m]: 23 µm
Total Size-Width: 133.69 µm
Total Size-Height: 134.91 µm
Total Size-Depth: 6.48 µm
Voxel-Width: 42.0 nm (pixel size)
Voxel-Height: 42.0 nm (pixel size)
Voxel-Depth: 80.0 nm
Resolution: 23.5938 pixels per micron
Zoom: 1.0
Objective: Plan-Apochromat 63x/1.40 Oil DIC

Numerical aperture: 1.40